The HUSERMET Project
the human serum metabolome in health and disease...



LC-MS: A Liquid Chromatography (LC) instrument attached to a Mass Spectrometer (MS).

Liquid Chromatography:

  • Samples are swept through the column by a constant flow of liquid (solvents or water)
  • The compounds within the sample are separated as they pass through, or interact with, the mesh inside the column
  • As the composition of the liquid changes (from less to more solvent) different compounds are released (eluted) from the column
  • As the compounds are released they are analysed using Mass Spectrometry

This technique allows complex biological samples to be separated and analysed effectively.

  • Low flow rates can be used to separate samples more slowly and increase the ability to detect low concentration compounds more accurately
  • Metabolites can be quantified by this method
  • Ultra Performance Liquid Chromatography (UPLC) offers increased resolution, sensitivity and speed over standard LC


The standard operating procedure (SOP) used for LCMS analysis in the HUSERMET study is available here. If you use this SOP, please cite the following publications:

[25 Sep. 2012]
HUSERMET represented at Metabomeeting 2012
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[30 Jun. 2011]
New Publication in Nature Protocols
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[23 Jan. 2010]
Manchester Researcher Appointed New 'Dementia Tsar'
[Read More...]
[04 Jan. 2010]
Newly Appointed Data Analyst
[Read More...]
[01 Nov. 2009]
Recruitment Finished
[Read More...]
[01 Aug. 2009]
Two New Publications
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[14 Apr. 2009]
Newly Appointed Research Technician
[Read More...]
[19 Nov. 2008]
New Project Manager
[Read More...]
[12 Sept. 2008]
HUSERMET Researchers Look to the Metabolome for Answers
[Read More...]
[07 Jul. 2008]
The University of Manchester and GSK Strike New Research Alliance
[Read More...]
[07 Jul. 2008]
Prof Doug Kell is the New Chief Executive for BBSRC
[Read More...]
[10 Dec. 2007]
The Maturing Field of Metabolomics is Taking Steps Toward Guiding Personalized Nutrition
[Read More...]
[24 Aug. 2007]
Free Health 'MOTs' in World's Biggest Male Ageing Study
[Read More...]
[20 Feb. 2007]
Newly Appointed Bioanalytical Scientist for the HUSERMET Project
[Read More...]